FIG 7.
Immunostimulatory potential of PAO1 sublines. (A and B) HEK reporter cells expressing human TLR4 (hTLR4) (A) or murine TLR4 (mTLR4) (B) were stimulated with a 5-log range of LPS concentrations, and NF-κB activation was recorded. (C to F) Murine cell lines MH-S and RAW 246.7 (C and D) and human cell lines A549 and THP-1 (E and F) were stimulated with 106 heat-killed whole-cell preparations of each strain. IL-6 and IL-1β were quantified from the cell supernatant using ELISA after 16 h of stimulation.