FIG 1.

Estrogen-related receptor alpha (ERRα) targets angiogenic genes in endothelial cells. (A) ERRα, ERRβ, and ERRγ mRNA expression in different ECs (n = 3). (B and C) ERRα protein expression in ECs (n = 3). (D and E) ERRα mRNA (D) and protein expression (E) in ERRα-KO compared to WT mouse lung ECs (n = 3). ****, P < 0.00005 by unpaired Student's t test. (F) Heat map representing differentially expressed genes from the microarray analysis in ERRα-KO and WT ECs. Differentially expressed genes were defined as having an absolute fold change of ≥2 and a P value of <0.05 (Bonferroni’s multiple-comparison test). The color bar on the left indicates the direction of differentially expressed genes (green, upregulated; red, downregulated). (G) GO term enrichment was calculated for differentially expressed genes using Cluster Profiler. The 10 most significant categories are shown. Each GO term is represented as a fraction of genes associated with a given GO term that were differentially expressed in ERRα-KO versus WT cells (x axis). The size of the circle represents the number of genes in the GO term, which were differentially expressed. The color of the circles represents the adjusted P value.