FIG 8.

Regulation of ERRα expression and schematic model. (A) ERRα mRNA expression in untreated, VEGFA (30 ng/ml)-treated, and hypoxia-treated HUVEC (n = 3). (B and C) Representative images of ERRα protein expression in VEGFA-treated HUVEC (B) and hypoxia-treated HUVEC (C) (n = 3). (D and E) Densitometry of Western blots quantifying ERRα expression in VEGF-treated (D) and hypoxia-treated (E) HUVEC. (F) ERRα expression in lung endothelial cells isolated from control and diabetic (high-fat diet plus STZ) mice (n = 5, technical replicates from pooled lungs). (G) Schematic model depicting regulation of angiogenesis by ERRα in ECs. ERRα acts as a transcriptional repressor of angiogenic genes and restricts angiogenesis. Proangiogenic stimuli have a dual effect on ERRα, decreasing its expression as well as increasing recruitment to angiogenic genes.