FIG 3.

LXRα/β ligand-induced binding and histone H3 acetylation in the cis-acting regulatory regions of known LXR target genes. (A) LXR occupancy was detected in the regulatory sites of known target genes in iBMDM macrophages using anti-FLAG antibody. Data are expressed as mean (±SD) values from three independent experiments. Asterisks indicate statistical significance relative to an irrelevant distal region: *, P < 0.05; **, P < 0.01. (B) LXRα/β binding capacity to LXR regulatory sites was tested in cells cultured with GW3965 and GW233 (24 h, 1 μM). Data are expressed as mean (±SD) values from two independent experiments. (C) Acetylation/deacetylation dynamics of histone H3 (H3K27ac) upon iBMDM treatment with GW3965 and GW233 was examined by ChIP-qPCR. Statistical significance was calculated between treatments in each iBMDM-LXR cell line with unpaired Student´s t test. *, P < 0.05; **, P < 0.01.