Table 2.

Comparison of amino acid mutation rates between catalytic and non‐catalytic sites of neuraminidase genes

Virus	Season	No. of samples	All sites			Catalytic sites			Non‐catalytic sites
			No. of total AA mutations	No. of total AAs	Mutation rate (%)	No. of total AA mutations	No. of total AAs	Mutation rate (%)	No. of total AA mutations	No. of total AAs	Mutation rate (%)	P value
A/H1N1pdm09	2013‐2014	20	49	9380	0.52	2	380	0.53	47	9000	0.52	0.991
	2015‐2016	20	42	9380	0.45	3	380	0.79	39	9000	0.43	0.308
	2016‐2017	9	14	4221	0.33	0	171	0.00	14	4050	0.35	0.441
	Total	49	105	22 981	0.46	5	931	0.54	100	22 050	0.45	0.711
A/H3N2	2011‐2012	48	120	22 512	0.53	3	912	0.33	117	21 600	0.54	0.387
	2012‐2013	48	97	22 512	0.43	4	912	0.44	93	21 600	0.43	0.971
	2013‐2014	20	77	9380	0.82	1	380	0.26	76	9000	0.84	0.219
	2014‐2015	40	165	18 760	0.88	0	760	0.00	165	18 000	0.92	0.008
	2015‐2016	19	58	8911	0.65	1	361	0.28	57	8550	0.67	0.367
	2016‐2017	76	226	35 644	0.63	4	1444	0.28	222	34 200	0.65	0.081
	Total	251	743	117 719	0.63	13	4769	0.27	730	112 950	0.65	0.001
B	2013‐2014	20	82	9320	0.88	0	380	0.00	82	8940	0.92	0.061
	2014‐2015	19	41	8854	0.46	0	361	0.00	41	8493	0.48	0.186
	2015‐2016	20	29	9320	0.31	0	380	0.00	29	8940	0.32	0.266
	2016‐2017	21	98	9786	1.00	0	399	0.00	98	9387	1.04	0.040
	Total	80	250	37 280	0.67	0	1520	0.00	250	35 760	0.70	0.001

AA, amino acid.

The neuraminidase AA lengths of A/H1N1pdm09, A/H3N2, and B viruses were 469, 469, and 466 AAs, respectively. The neuraminidase catalytic sites consisted of a total of 19 AAs.