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On-target/off-target mutagenesis in HeLa and HepG2 cells. (A, B) On-target indel frequencies at VEGFA1 site in HeLa and HepG2 cells. (C, D) Off-target indel frequencies at OT-1 site in HeLa and HepG2 cells. (E, F) Off-target indel frequencies at OT-2 site in HeLa and HepG2 cells. We transfected HeLa and HepG2 cells as in Figure 2 and analyzed the indel frequencies as in Figure 3. The data represent the averages ± SD of three independent culture experiments.
