. 2018 Nov 20;47(3):1468–1481. doi: 10.1093/nar/gky1171

Table 2.

Relative triplex thermostability and folded population

	T _m,1 (°C)			E _FRET
	^aM1^ET	^aM1^AB	^bM1^TH	M1^ET	M1^AB
Maximum signal	66.6	57.1	78.5	0.81	0.55
Minimum signal	38.4	35.2	59.2	0.57	0.23
Signal in near-physiological ionic conditions	53.2	48.8	63.8	0.75	0.46
Relative^c stability (T_m,1) or folded population (E_FRET)	52%	62%	24%	75%	72%

^a T _m,1 calculated using DS-FRET assay.

^b T _m,1 calculated using DSF assay.

^cRelative thermostability or folded population was calculated using [(P – Min)/(Max – Min)] × 100, where Max, Min and P indicate maximum signal, minimum signal, and the signal in near-physiological ionic conditions (∼150 mM monovalent, 1 mM MgCl₂), respectively. P was calculated from the average of the signal obtained in 102.6 mM and 202.6 mM monovalent concentrations.