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. 2018 Nov 20;47(3):e16. doi: 10.1093/nar/gky1173

Figure 1.

Figure 1.

scFTD-seq platform and workflow. (A) Microwell array devices used in scFTD-seq method. For closed-environment format, microwell arrays are bonded to a microfluidic channel. For open-surface format, microwell arrays are used without a channel on top. (B) Cell and bead capture in microwell arrays before (top) and after (bottom) freeze-thaw cell lysis. (C) Open-surface format workflow for scFTD-seq. (D) Closed-environment format workflow for scFTD-seq. (E) Schematic of the scFTD-seq workflow with distributed sampling and modular operation. Library preparation after freeze-thaw lysis follows the protocol described in DropSeq method (17).