Figure 4.

Inferring cell types in heterogeneous samples—whole tumor. (A) t-SNE plot of cells dissociated from melanoma tumor generated in YUMMER1.7 syngeneic mouse model in which tumor cells express Gfp. Two major clusters are identified where the left cluster is melanoma cells inferred by Gfp expression. (B) Immune cell cluster is inferred by Ptprc (Cd45) expression. (C) Unsupervised clustering analysis identified 4 clusters where clusters 1 (n = 337), 3 (n = 56) and 4 (n = 12) were identified as immune cells, and cluster 2 (n = 237) as tumor cells. (D) Differential gene expression analysis between the identified clusters.