Table 3.
Metrics of single DNA molecule packaging dynamics with wild type terminase (WT) and mutants determined by analysis of the optical tweezers measurements. Results for Walker A mutants V80A and R79K obtained previously (41) are shown for comparison. Except where indicated all measurements were recorded with 500 μM ATP. Quantities are reported as averages over all events and reported uncertainties are standard errors in the means
| Pauses | |||||
|---|---|---|---|---|---|
| Enzyme | Events | Motor velocity (bp/s) | Slip frequency (slips/kb) | Frequency (pauses/kb) | Duration (s) |
| WT | 53 | 400 ± 20 | 0.5 ± 0.1 | 1.2 ± 0.4 | 2 ± 0.1 |
| WT 5 μM ATP | 9 | 130 ± 20 | 5 ± 1 | 8 ± 2 | 1.6 ± 0.1 |
| WT 2.5 μM ATP | 13 | 79 ± 6 | 7 ± 1 | 13 ± 2 | 1.8 ± 0.1 |
| E179A | 0 | - | - | - | - |
| E179Q | 0 | - | - | - | - |
| E179D | 25 | 50 ± 20 | 1.6 ± 0.5 | 24 ± 2 | 3.3 ± 0.2 |
| R79K | 5 | 42 ± 3 | 1.3 ± 0.5 | 24 ± 2 | 3.4 ± 0.3 |
| V80A | 51 | 230 ± 10 | 3 ± 1 | 5 ± 1 | 4.4 ± 0.3 |