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. 2018 Nov 22;47(3):1505–1522. doi: 10.1093/nar/gky1190

Figure 1.

Figure 1.

Meg3 expression is induced by the DNA damage response in a p53 dependent manner. (A) qPCR analysis of Meg3 expression in HUVECs treated with 200 nM, 600 nM and 1 μM of doxorubicin for 12 h. (B) qPCR analysis of Meg3 expression in HUVECs treated with 10 μM nutlin-3 for 12, 24 and 48 h. (C) qPCR analysis of Meg3 expression in HUVECs treated with 100 μM palmitic acid for 12, 24 and 48 h. (D) qPCR analysis of Meg3 expression in HUVECs treated with doxorubicin (40 nM for 12 h) or nutlin-3 (10 μM for 12 h) with or without lentiviral knockdown of p53. (E) In situ hybridization of Meg3 in HUVECs treated with or without 0.2 μM doxorubicin for 12 h. The number of red dots per nucleus from 13 cells was counted for each condition. Data show mean ± S.E.M., n = 3 (A–D), n = 13 (E); *P < 0.05.