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. 2018 Nov 22;47(3):1505–1522. doi: 10.1093/nar/gky1190

Figure 5.

Figure 5.

Meg3 knockdown promotes apoptosis and inhibits proliferation in ECs. HUVECs were transfected with control or Meg3 GapmeRs, treated with or without 10 ng/ml TNF-α for 12 or 16 h. (A) Caspase 3/7 activities reflected by luminescence levels were measured, and fold changes were calculated relative to that in ECs transfected with control GapmeRs without TNF-α treatment. (B) The number of TUNEL positive cells per high power view were shown. TUNEL positive cells are in red color due to presence of DNA breaks, and DAPI-stained nuclei appear in blue. (C) The percentages of EdU positive cells (red) among Hoechst 33342 (blue) stained cells were calculated in transfected cells with or without 12 h TNF-α treatment. (D) Cell cycle distribution revealed by flow cytometry. Data show mean ± S.E.M., n = 3; *P < 0.05.