Figure 2.

NHEJ under stress conditions. Similar experiments as in Figure 1 were performed using competent cells prepared from stationary phase cultures (A; green) or exponential phase cultures after a 75min shift from 28 to 40°C (B; red). Values obtained with the WT strain in exponential phase at 28°C (blue) are the same as in Figure 1 and are reported as a reference. Results shown are the means (±SEM) of the plotted values, obtained from independent experiments (n ≥ 3). The absence of value means that no colony was obtained (detection threshold indicated on the graph). Statistical analyses were performed using a one-way analysis of variance (see Supplementary Table S4 for detailed statistical analysis results). The Bonferroni posttest was used to carry out the indicated comparisons (*P< 0.05; ***P < 0.001).