Figure 1.

DNA gyrase structure and Topo-Seq procedure. (A) DNA gyrase structure with gyrase domains (GHKL, TOPRIM, WHD, CC, CTD) indicated. (B) Topo-Seq workflow: (i) Schematic illustration of DNA gyrase on DNA plectonemes; (ii) A gyrase-DNA complex trapped with an inhibitor, here quinolone-like (red stars); (iii) C-terminal SPA tag is recognized by M2 antibodies and used to precipitate gyrase-bound fragments; (iv) Deproteinized DNA fragments have blocked 5′-ends; (v) Resulting signals visualized.