Figure 4.

LIG interactions with ATP•Mn²⁺ and surface electrostatics. (A) Stereo view of the active site highlighting contacts to the ATP phosphates, two associated manganese ions (green spheres), and a nearby sulfate anion (stick model). Amino acids and ATP are shown as stick models with beige and gray carbons, respectively. Waters are depicted as red spheres. Atomic contacts are indicated by black dashed lines. (B) Stereo view of a surface electrostatic model of Trl1-LIG (generated in Pymol). ATP in the active site and a nearby sulfate anion are depicted as a stick models. Manganese ions are green spheres.