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. 2018 Dec 24;47(3):1440–1450. doi: 10.1093/nar/gky1277

Figure 4.

Figure 4.

Viscosity-adjusted rate constants as a function of crowder’s volume occupancy. Viscosity-adjusted rate constants were calculated according to Supplementary Equation S6 (using results reported in Figure 3). (A and B) Small crowders (< PEG 600) do not affect transcription kinetics (excluding effect of viscosity). (A) Large PEGs (PEG 600∼8000) show strong dependence on size and volume occupancy. (B) Although large polysaccharide crowders (Dextran 5, 10, 500, Ficoll70) show dependence on size and volume occupancy, the trend of size dependence is non-monotonic, which is different from large PEGs. Zoomed-in curves for Dextran 5 (orange hollow circle), Dextran 10 (teal star), Ficoll 70 (violet sphere) and Dextran500 (green hexagon) are shown in the inset. Errors are propagated according to Supplementary Equation S6. (C) Linear fits to all curves in (A) and (B) against crowders were performed to show qualitatively the trend of transcription kinetics as a function of crowder size. Lines are guides to the eye.