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Systemic biodistribution and tissue accumulation of lipid-hsiRNA conjugates. Biodistribution of lipid-hsiRNAs 48 h after a single, subcutaneous injection (n = 3 mice, 20 mg kg−1). (A) Kidney and liver distribution of lipid-conjugated hsiRNAs. Cy3-labeled lipid-hsiRNAs (red), nuclei stained with DAPI (blue). (B) Guide strand quantification of Cy3-labeled lipid-hsiRNAs by a PNA hybridization-based assay. Data presented as mean ± SD.
