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Thermal stability of TP3-T6 and mutated sequences
| Name | Sequence (5′ → 3′) | T m (°C)a |
|---|---|---|
| TP3-T6 | TGGGGTCCGAGGCGGGGCTTGGG | 60.2 ± 0.9 |
| TP3-T6-[I14] | TGGGGTCCGAGGCIGGGCTTGGG | 57.3 ± 0.6 |
| TP3-T6-[I2] | TIGGGTCCGAGGCGGGGCTTGGG | 60.2 ± 0.7 |
| TP3-T6-[T14, A2] | TAGGGTCCGAGGCTGGGCTTGGG | 63.6 ± 0.8 |
aMelting temperature (Tm) was measured in 70 mM KCl and 20 mM KPi (pH 7).
