Figure 2.

(A) Illustration of OR gate. The fluorophore FAM and the quencher BHQ are functionalized at either end of substrate strand R1. (B) Time-dependent normalized fluorescence changes (ΔF/MaxΔF) during 2-h reaction process. The sample interval was 3 min. Curves (1) to (4) demonstrate the gate responses to different inputs. Here, symbol + denotes the addition of strand and symbol—denotes the absence of strand. All data represent the average of three replicates. Error bars represent one standard deviation from triplicate analyses. (C) Illustration of AND gate. The fluorophore FAM and the quencher BHQ are functionalized at either end of substrate strand R1. (D) Time-dependent normalized fluorescence changes (ΔF/MaxΔF) during 2-h reaction process. The sample interval was 3 min. Curves (1) to (4) demonstrate the gate responses to different inputs. Here, symbol + denotes addition of the strand and symbol − denotes absence of the strand. All data represent the average of three replicates. Error bars represent one standard deviation from triplicate analyses. (E) Time-dependent changes of concentrations of reactants during OR logic operation triggered by only one input I2. Curves (1)–(3) denote time-dependent changes of concentrations of input strand I2, output O1 and OR gate complex Z1/T2/R1, respectively. (F) Time-dependent changes of concentration of reactants during OR logic operation triggered by both inputs I2 and I3. Curves (1)–(3) denote time-dependent changes of concentrations of input strands I2 and I3, output strand O1 and OR gate complex Z1/T2/R1, respectively. (G) Time-dependent changes of concentrations of reactants during AND logic operation triggered by both inputs I4 and I5. Curves (1)–(4) denote time-dependent changes of concentrations of input strands I4, I5, output strand O1 and AND gate complex Z2/T3/T4/R1, respectively.