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. 2018 Dec 19;47(3):1416–1427. doi: 10.1093/nar/gky1271

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© The Author(s) 2018. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 3. — Protection of cleavage sites by NAPs. (A and C) UCSC browser view in higher magnification for 3905–4050 and 5050–5200 kb genomic coordinates respectively. (B and D) Cleavage assay with DNA having TopoI recognition motif but cleavage not seen in vivo. Oligonucleotides (32mer) from DNA binding peaks were treated with increasing concentration of MsTopoI (0.01–0.16 μM). The resultant products were analysed on 8M urea 12% polyacrylamide gel. (E and F) Protection assay with NAPs. 32mer oligonucleotides were incubated with increasing concentration of HU (E) or Lsr2 (F) and cleavage assays were carried out with TopoI (0.2 μM). The resultant products were analysed on 8 M urea and 12% polyacrylamide gel.