Figure 5.

Loss of Pds5 does not alter cohesin loading on chromatin. (A) Levels of Rad21 bound to chromatin after depletion of Pds5A (top) or Pds5B (bottom). Chromatin Fractionation of Pds5 depleted U-2 OS cells. Fractions separated included whole cell, chromatin bound and soluble cytoplasmic proteins. Samples were probed with Pds5A, Pds5B, Rad21, Tubulin and H3 antibodies. (B) Relative intensity of Rad21 bound to chromatin after immunofluorescence staining of Pds5A or Pds5B depleted cells. Cells were pre-extracted to remove soluble proteins, leaving chromatin bound proteins intact. Quantification of the Rad21 relative intensity signal. At least 100 cells were scored for each data set. n = 2. Data are represented as mean ± SEM. Statistics: Mann–Whitney; ****P < 0.0001.