Table 1. Information on the primers used for RT-PCR.
Genes | Access No. | Forward primer (5’-3’) | Reverse primer (5’-3’) | Tm | Amplicon size, bp |
---|---|---|---|---|---|
(°C) | |||||
GAPDH | BC023196 | CATCACCATCTTCCAGGAGCG | GAGGGGCCATCCACAGTCTTC | 59 | 357 |
HMOX1 | NM010442 | CAGGTGATGCTGACAGAGGA | ACAGGAAGCTGAGAGTGAGG | 62 | 184 |
CAT | NM009804 | GGAGGCGGGAACCCAATAG | GTGTGCCATCTCGTCAGTGAA | 59 | 102 |
GPX | NM008160 | GTGCGAAGTGAATGGTGAGA | CTGGGACAGCAGGGTTTCTA | 59 | 253 |
SOD | NM011434 | TTCGAGCAGAAGGCAAGCGGTGAA | AATCCCAATCACACCACAAGCCAA | 59 | 396 |
BAX | NM007527 | CCAGGATGCGTCCACCAA | AAGTAGAAGAGGGCAACCAC | 62 | 195 |
BCL2 | NM007527 | AACTCTTCAGGGATGGGG | GCCGGTTCAGGTACTCAG | 59 | 144 |
CASP-3 | NM001284409 | GGAGAAATTCAAAGGACGG | AACAAAACAGAAACACGCCC | 59 | 260 |