Table 3. In vitro development of mouse embryos with different concentrations of H2O2.
Treated groups | No. of zygotes cultured † |
Percentages of embryos developed to [mean (SEM), %] |
||
---|---|---|---|---|
2-cell | 4-cell | Blastocyst | ||
Control | 125 | 96.2 ± 0.87 a | 82.3 ± 1.43 a | 45.0 ± 1.10 a |
H2O2 (5 μM) | 127 | 88.2 ± 1.02 a | 78.2 ± 1.96 a | 38.2 ± 0.80 a |
H2O2 (10 μM) | 126 | 89.3 ± 1.30 a | 62.0 ± 2.00 b | 23.0 ± 1.73 b |
H2O2 (20 μM) | 125 | 86.4 ± 1.10 a | 57.5 ± 1.77 c | 10.0 ± 0.72 c |
a–c Values within a column with different superscripts significantly differ (P < 0.05). † Experiment was replicated more than 5 times.