Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Spindle shape and chromosome alignment in mouse MII oocytes. (A) Fresh oocytes, which were immediately collected at 15 hours after hCG administration. (B, C) Aged oocytes, which were cultured for 12 h in medium following oocyte collection. (D–F) Higher magnified images of spindle (left) and chromosome alignment (right). White arrowheads indicate chromosome misalignment. Immunofluorescent staining of α-tubulin (green). DNA (blue) was stained with DAPI. Scale bar = 10 μm.
