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. 2018 Nov 22;65(1):57–66. doi: 10.1262/jrd.2018-056

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©2019 Society for Reproduction and Development

This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/)

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Fig. 4. — Localization and expression level of MAD2 in fresh mouse oocytes with blocked microtubule polymerization. (A) Immunofluorescent staining of MAD2 (red) and α-tubulin (green). DNA (blue) was stained with DAPI. D-PBS, instead of the primary antibody, was used as the negative control. The inserted panel in the middle panel of the nocodazole group indicates only MAD2 signals. Scale bar = 10 μm. (B) Western blotting and expression levels of MAD2 in oocytes treated with nocodazole. β-actin was used as a loading control.