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. 2017 Mar 8;15(2):133–141. doi: 10.5301/jabfm.5000338

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© 2017 The Authors

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs 4.0 License (http://www.creativecommons.org/licenses/by-nc-nd/4.0/) which permits non-commercial use, reproduction and distribution of the work as published without adaptation or alteration, without further permission provided the original work is attributed as specified on the SAGE and Open Access page (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Fig. 4 — Mesenchymal stem cell (MSC) quantification after 1, 7 and 14 days of culture in the scaffolds. Cell nuclei were stained with DAPI (blue) and analyzed by laser scanning confocal microscopy (LSCM). The 3D reconstructions of poly(lactic-co-glycolic acid) (PLGA) pure scaffold merged with adhered cells are presented at the bottom of the image. 3D reconstructions of PLGA/simvastatin (SIM) with cells were not carried out due to SIM autofluorescence, blurring the visualization of the cells. Also, 3D reconstructions of PLGA after 1 day in culture were not performed because there were few cells to justify the reconstruction. The cell count graph represents the median and standard deviation of a triplicate assay.