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Removing the aneuploidy on chromosome III decreases ethanol tolerance. Drop test assays of strains 2-200-2 and 2-200-2-S4 in ethanol plates. Plates were incubated 10 days at 28°C. 2-200-2 had three copies of chromosome III and 2-200-2-S4 had two copies. Removing the aneuploidy affects ethanol tolerance.
