Table 1.
Gene | NCBI accession no. | Forward 5′–3′ | Reverse 5′–3′ | TA | Product (bp) |
---|---|---|---|---|---|
Ptgs1 | NM_008969.4 | ATTGCACATCCATCCACTCCC | AGTTGTCGAGGCCAAAGCG | 62 | 196 |
Ptgs2 | NM_011198.4 | CTTTGCCCAGCACTTCACCC | GGGGATACACCTCTCCACCAA | 64 | 191 |
Cybb | NM_007807.5 | AAGTTCGCTGGAAACCCTCC | GCCAAAACCGAACCAACCTC | 62 | 88 |
Il6 | NM_031168.2 | ACTTCACAAGTCGGAGGCTT | GGTACTCCAGAAGACCAGAGG | 64 | 220 |
Zc3h12a | NM_153159.2 | CAGCCTCGACCAGATGTGCC | CAGCCGCTCCTCGATGAAGC | 62 | 237 |
Ef2 | NM_001961 | GACATCACCAAGGGTGTGCAG | TTCAGCACACTGGCATAGAGGC | 62 | 214 |
TA, annealing temperature.