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. 2019 Feb 12;10:6. doi: 10.3389/fphys.2019.00006

Table 1.

Sequences of primers used in qRT-PCR analysis of gene expression.

Gene NCBI accession no. Forward 5′–3′ Reverse 5′–3′ TA Product (bp)
Ptgs1 NM_008969.4 ATTGCACATCCATCCACTCCC AGTTGTCGAGGCCAAAGCG 62 196
Ptgs2 NM_011198.4 CTTTGCCCAGCACTTCACCC GGGGATACACCTCTCCACCAA 64 191
Cybb NM_007807.5 AAGTTCGCTGGAAACCCTCC GCCAAAACCGAACCAACCTC 62 88
Il6 NM_031168.2 ACTTCACAAGTCGGAGGCTT GGTACTCCAGAAGACCAGAGG 64 220
Zc3h12a NM_153159.2 CAGCCTCGACCAGATGTGCC CAGCCGCTCCTCGATGAAGC 62 237
Ef2 NM_001961 GACATCACCAAGGGTGTGCAG TTCAGCACACTGGCATAGAGGC 62 214

TA, annealing temperature.