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. Author manuscript; available in PMC: 2019 Feb 19.
Published in final edited form as: Methods Mol Biol. 2019;1869:105–116. doi: 10.1007/978-1-4939-8805-1_10

Fig. 1.

Fig. 1

Spot Assay: The assessment of migration is dependent on the distance traveled from the middle spot of which the cells were dropped. The principal relies on placing a polycarbonate filter in the middle of a well and dropping 1ul of cell concentration on the top of the filter. Over time, the cell of interest will migrate away from the point of origin whereby the filter is then fixed, imaged and analyzed for distance cells traveled in response to exogenous supplements. Adapted from: Dose-dependent effect of EGF on migration and differentiation of adult subventricular zone astrocytes. Perez-Gonzalez O, Quinones-HinojosaA. Glia.58 (8),2010. Pages:975–983