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. 2019 Feb 18;19:158. doi: 10.1186/s12885-019-5363-4

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — a GnRH secretion in static incubation from media of COS in vitro treated for 4 h with 17β-estradiol (100.0 pM), kisspeptin-10 (10.0 nM), or a combination of E2 and kisspeptin-10. b Representative plot of GnRH secretion from perifused COS treated with either 0.05% DMSO vehicle or 10.0 nM kisspeptin-10. Lines indicate duration of exposure to drug/vehicle