Fig. 6.

TSA inhibits EGF-induced HNSCC cell invasion through suppressing the EGFR-Arf1 complex. a, b The effect of TSA on EGF-induced Arf1 activation. Representative images were shown in (a) and quantitative data from three independent experiments were shown in (b). c The effect of the Arf1 inhibitor Exo2 on EGF-induced signaling pathways. d The effects of TSA and Exo2 on EGF-induced HNSCC cell invasion measured by Borden chambers pre-coated with Matrigel. In these assays, EGF at 50 ng/ml, TSA at 5 μM, or Exo2 at 20 μM was used to treat HN12 and HN31 cells. **p < 0.01