Fig. 2.

T-cell repertoire is detected from the extracted DBS RNA. a uCDR3 discovery from different amounts of punch disks (**p < 0.01, by Student’s t-test). b TCR repertoire diversity is illustrated in tree maps where each rounded rectangle represents a unique entry: V–J–uCDR3 and the size of the spot denotes the relative frequency. c The relative frequency from low (black) to high (red) of V–J gene combinations, is illustrated in the 2D heat maps from varying amounts of punch disks (left to right: 3, 10, 40 and 80 disks). ns indicates no significant statistical difference