Table 1.
Diversity | Low | Moderate | High | |||
---|---|---|---|---|---|---|
Sample | WB | DBS | WB | DBS | WB | DBS |
Duplicate sequence repeatability (R2) | 0.994 | 0.991 | 0.993 | 0.956 | 0.930 | 0.699 |
Unique CDR3 | 43,653 | 5327 | 38,431 | 3820 | 25,118 | 3208 |
D50 | 0.3 | 1.1 | 2.7 | 7.6 | 14.5 | 6.8 |
Diversity Index | 15.8 | 13.2 | 20.6 | 16.8 | 27.1 | 11.1 |
R2 of regression between DBS and WB | 0.989 | 0.865 | 0.806 | |||
Top 50 dominant clones (%)a | 94% | 98% | 82% | |||
Top 100 dominant clones (%)a | 92% | 87% | 74% |
WB whole blood, DBS dry blood spot, R2 coefficient of determination calculated by linear regression
aThe percentage of TCR dominant clones from whole blood could be found from DSB examination