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Generation of third-generation CAR T cells targeting MSLN. a Schematic diagram of the M28z10 transgene. b Percentage of GFP and M28z10 transduced primary human T cells detected by flow cytometry. c Representative graph of the expansion rate of M28z10 CAR T cells in 10 days. d Detection of CCR7, CD62L, CD45RA, and CD45RO on the manufactured T cells. e Detection of exhaustion markers, including TIM-3, LAG-3, and PD-1 on the manufactured T cells
