Fig. 1.

Iron-induced metabolic dysregulation is dependent on genetic background. High iron-fed LG/J mice have decreased body weight (a), caused in part by decreased adiposity (b), which is not seen in high iron-fed SM/J mice. Loss of adipose tissue is associated with altered glucose metabolism, assessed by intraperitoneal glucose tolerance test (c), summarized by increased area under the curve (d). Decreased glucose clearance in LG/J mice is associated with increased blood glucose (e) and decreased serum insulin (f). Panels a, b: n = 16–19 mice per strain X diet cohort. Panels c, d, e: n = 15–18 mice per strain X diet cohort. Panel f: n = 12–17 mice per strain X diet cohort. Panel A - *p-value < 0.05 assessed by students t-test. Panels B, D, E, F - *p-value < 0.05, **p-value < 0.01, assessed by ANOVA with Tukey’s Post Hoc Correction. N.S. – Not significant. Error bars represent standard deviation