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. 2019 Feb 18;14:54. doi: 10.1186/s13018-019-1098-9

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — rBMSC characterization. An example of rBMSCs phenotype and functional analysis. a Flow cytometry analysis of MSCs for surface markers (CD45⁺ isotype control, CD90⁺ isotype control, negative control) showed that isolated bone marrow-derived cells expressed CD90, but not the hematopoietic marker CD45, confirming that only mesenchymal cells had been isolated (CD45⁻CD90⁺ cells population). b–d In vitro differentiation of mesenchymal cells: the cells were incubated in lineage-specific induction media and then analyzed by histochemical and cytological staining for b adipocytes (Oil Red-O staining), c osteoblasts (alizarin red staining), and d chondrocytes (Alcian Blue staining)