Fig. 5.
Monocyte and macrophage markers in tibialis anterior and gastrocnemius muscle are not altered between isotype control and anti-HMGB1 antibody-treated SOD1G93A mice. SOD1G93A mice were intraperitoneally injected weekly with the anti-HMGB1 antibody at 35 days of age (100 μg). Monocytes and macrophage markers in tibialis anterior (TA) and gastrocnemius (GN) muscle of vehicle and anti-HMGB1-treated SOD1G93A mice was investigated at mid-symptomatic stage (133 days) using quantitative PCR. a–c Shows anti-HMGB1 treatment had no effect on macrophage (Itgam, Cd68 and Aif1) mRNA transcript levels in both TA and GN muscles (n = 6, p > 0.05, Student’s t test). d Shows a reduction in monocyte (Ly6c) mRNA transcript levels in TA muscle of anti-HMGB1-treated SOD1G93A mice, while no change was evident in GN muscle when compared to isotype control-treated SOD1G93A mice (n = 6, *p < 0.05, Student’s t test). Data are presented as mean ± SEM