Fig. 4.

ROS decreases the expression of Stathmin1 and blocks the interaction between PI3K and Stathmin1. a Western blot of acetyl α-tubulin^K40, PI3K and P85 (loading control) in hypoxia HUVEC cells expressing PIK3CA-WT, PIK3CA-H1047R and E545K or treated with PIK3CA siRNA. b Western blot of Stathmin1 siRNA-treated HUVEC cell using antibodies to Stathmin1 and β-actin (loading control). c HUVEC cells were transfected with Stathmin1 siRNA or Stathmin1 expression vector and treated with or without 1 μM or 10 μM wortmannin, total lysates were immunoblotted with antibodies to acetyl α-tubulin^K40, Stathmin1 and P85. d Immunoprecipitation of PI3K from normoxia, hypoxia and H₂O₂ treated HUVEC cells using endogenous protein and immunoblotted for Stathmin1 and PI3K. Representative results from three independent experiments were depicted here. The relative intensity of band was normalized to P85. The data are presented as the mean ± SEM. *P < 0.05