Table 2.

Primer sequences and PCR amplification parameters of reference genes and target genes derived from RT-qPCR analysis.

			Product	Amplification	Mean	Standard		Rank
Genes	Forward primer	Reverse primer	length (bp)	efficiency (%)	Cq	Deviation (SD)	CV%	by CV
UBQ1	GTGCTCATTAAAGTCCGTACCGG	TACCTGTCCACCTACAACCACAG	126	2.07	22.48	0.52	2.31	4
COG	CAGCACGAAGAAAAGGAACGCG	CGGTATAAGACGACGACATTGAC	104	1.99	23.12	0.47	2.03	1
TMN	GACAGATCCCAGAACAAGTGC	TGGTGGGACCATATTGAGTTCAG	124	2.06	20.78	0.45	2.16	2
CDC42	GTGCTCTGCCCTTACTCAACG	CAACTGAGCAACCGCCCTTTT	104	1.98	21.69	0.70	3.23	12
ARL	CTACAAGTTGGTGAGGTGGTG	ACTGGTCTGTCCGCCTAAATC	111	2.01	21.92	0.59	2.69	8
TMED	GCCAGCAGGCAAGAAAGAATGT	CACGACCAGATGGAGCAGAAAT	128	1.97	21.77	0.57	2.62	7
RhoA	CCCAGAAGTCTACGTCCCAAC	TCCTGTCCTGCTGTATCCCAC	102	1.99	19.91	0.54	2.71	9
MOB	CCTAAATGAGTGGATCGCTGTG	TGGTATTCATACTTCGGTCCTGC	132	1.94	21.85	0.49	2.24	3
RPS15	GTGGAAGTCAAGCCGGAGAT	AATCTTGACGAGTGAGTGGCG	110	1.82	16.46	0.48	2.92	10
RPL17	CGGAGTTGGTCGTTGTGCC	CCTTGACATCGGCGTTGCT	123	1.83	16.49	0.39	2.36	5
UBQ2	CAAGATTCCCGAGACCTATCC	CAGCCCATTGGTCCTTGAGTAT	125	2.23	21.03	0.53	2.52	6
TubA	CGAGCAGTCTGTATGTTGAGC	TTCCTCCATTCCCTCACCG	129	2.11	19.37	0.62	3.20	11
TubB	TCTCCGAGCAGTTTACCGCTATG	GTACTCGCTCACCAAGTCGTTC	125	1.98	16.74	0.83	4.96	14
GAPDH	CAAGCCAGCAACTTACGACGAG	GTGTCGCCGTTGAAATCAGTC	123	2.00	19.28	0.84	4.36	13
BAG	TCGTGGAATCGAACCTGTCCTTG	CATGAGCTGTAGGTGCTTGGTG	116	1.98	–	–	–	–