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. 2019 Feb 19;476(4):629–643. doi: 10.1042/BCJ20180435

Figure 3. Phosphoglycolate accumulation blocks phosphoglycerate mutase — a process that can be prevented by expression of gpmI.

Figure 3.

(A,B) Schematic representations of part of glycolysis (A) and the reaction catalysed by phosphoglycerate mutase in the absence or presence of phosphoglycolate (B). (C,D) The ratios of 3-P-glycerate/2-P-glycerate concentrations (C) and phosphoglycolate concentrations (D) were assessed in parental HCT116 cells or in a PGP knockout clone expressing (or not) the 2,3-BPG-independent phosphoglycerate mutase, gpmI, from Escherichia coli. Values represent the mean ± s.e.m. of four independent experiments and are presented normalized to total ion current in arbitrary units. Asterisks indicate P < 0.05 in post hoc t-test analysis corrected for multiple testing.