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. 2019 Feb 2;13(1):88–101. doi: 10.1080/19336950.2019.1568824

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© 2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — Glutamate-induced Kv2.1 and VAP declustering in cultured hippocampal neurons. The effect of 20 μM glutamate on GFP-Kv2.1 and Ruby2-VAPA co-localization on the basal membrane of transfected DIV 8 rat hippocampal neurons is illustrated. Cells were transfected with GFP-Kv2.1 and Ruby2-VAPA and after 24 hrs imaged via TIRF microscopy. The top row illustrates the glutamate dephosphorylation-induced Kv2.1 declustering while the bottom row illustrates the VAP unbinding that occurs in parallel.