Table 2. Alignment of statistical results of reads.

Sample name	Control-1	Control-2	Control-3	igf3-dsRNA-1	igf3-dsRNA-2	igf3-dsRNA-3
Notes: Raw reads: The number of total reads. Clean reads: The number of clean reads. Clean base (G): The data of clean reads. Total mapped reads: The number of sequences matched to the genome. Multiple mapped reads: The number of sequences that had multiple positions mapped to the reference sequence. Uniquely mapped reads: The number of sequences that had unique positions mapped to the reference sequence. Read-1, read-2 mapped: The number of read-1 and read-2 that locate on the genome; the statistical proportion of the two parts should be substantially the same. Reads map to '+': The number of reads mapped to the positive strand of the genome. Reads map to '-': The number of reads mapped to the negative strand of the genome.
Raw reads	103838052	129702320	109996624	98107722	130263708	91249756
Clean reads	99410868	123563848	104194694	94321406	122675946	89307666
Clean base (G)	14.91	18.53	15.63	14.15	18.4	13.4
Total mapped reads	81530347(82.01%)	99404340(80.45%)	85870625(82.41%)	77049685(81.69%)	99493319(81.10%)	73064096(81.81%)
Multiple mapped	12853963(12.93%)	18112363(14.66%)	12915566(12.40%)	15084557(15.99%)	15762693(12.85%)	10390489(11.63%)
Uniquely mapped	68676384(69.08%)	81291977(65.79%)	72955059(70.02%)	61965128(65.70%)	83730626(68.25%)	62673607(70.18%)
Read-1 mapped	34516969(34.72%)	40477251(32.76%)	36673789(35.20%)	31095154(32.97%)	41919370(34.17%)	31496714(35.27%)
Read-2 mapped	34159415(34.36%)	40814726(33.03%)	36281270(34.82%)	30869974(32.73%)	41811256(34.08%)	31176893(34.91%)
Reads map to ‵+′	34383536(34.59%)	40550020(32.82%)	36580679(35.11%)	31056772(32.93%)	41822537(34.09%)	31350486(35.10%)
Reads map to ‵−′	34292848(34.50%)	40741957(32.97%)	36374380(34.91%)	30908356(32.77%)	41908089(34.16%)	31323121(35.07%)