Table 1. Statistics of DNA reads produced for the E. chlorotica genome in this study.

Platform	Insert size (bp)	No. of Libraries	Read length (bp)	Raw data										Clean data
				Total bases (Gb)	Sequencing coverage (X)	Physical coverage (X)	Total bases (Gb)	Sequencing coverage (X)	Physical coverage (X)
Note: Each of the five Illumina mate-pair libraries (2.5 kb × 2, 5 kb × 2 and 10 kb × 1) was run on two lanes with read length of 49 bp and 90 bp, respectively. Coverage calculation was based on the estimated genome size of 575 Mb according to k-mer analysis. Sequence coverage is the average number of times a base is read, while physical coverage is the average number of times a base is spanned by sequenced fragments.
Illumina	170	2	100	35.55	61.83	52.55	28.67	49.86	41.56
	500	2	100	32.44	56.42	141.06	18.12	31.51	84.04
	800	2	100	34.51	60.02	240.04	21.01	36.54	158.32
	2,500	2	49;90	76.60	133.22	1,752.62	43.77	76.12	1,301.05
	5,000	2	49;90	83.67	145.51	4,771.57	46.30	80.52	2,767.88
	10,000	1	49;90	33.97	59.08	3,841.34	18.45	32.09	2,174.84
	Total	11	—	296.73	516.05	10,799.18	176.32	306.64	6,527.68
PacBio	6,000	3	1,224	9.45	16.43	—	5.36	9.32	—