Table 2. Estimation of genome size and heterozygosity of E. chlorotica by k-mer analysis.

k	Total number of k-mers	Minimum coverage (X)	Number of erroneous k-mers	Homozygous peak	Estimated genome size (Mb)	Estimated heterozygosity (%)
Note: k-mer frequency distributions were generated by Jellyfish (v2.2.6) using 62.8 Gb Illumina clean data as input and a series of k values (17, 19, 21, 23, 25, 27, 29 and 31) with the -C setting. Minimum coverage was the coverage depth value of the first trough in k-mer frequency distribution. k-mers with coverage depth less than the minimum coverage were regarded as erroneous k-mers. Estimated genome size was calculated as (Total number of k-mers – Number of erroneous k-mers)/Homozygous peak.
17	51,187,863,592	13	1,410,585,877	86	579	3.59
19	49,735,232,800	11	1,804,614,643	84	571	3.93
21	48,282,601,880	11	2,010,206,114	80	578	3.90
23	46,829,970,960	11	2,152,586,758	78	573	3.79
25	45,377,340,040	10	2,235,304,391	75	575	3.69
27	43,924,709,120	10	2,327,591,479	72	578	3.57
29	42,472,078,200	9	2,370,847,012	70	573	3.47
31	41,019,447,280	9	2,433,307,151	67	576	3.36