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. 2019 Feb 12;13:26. doi: 10.3389/fncel.2019.00026

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Copyright © 2019 Forbes and Andrews.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Increasing expression of α9 integrin in iPSC-derived hNPCs promotes neurite outgrowth when grown on TN-C. Following transduction of hNPCs with either LV-α9-eYFP or LV-fGFP, cells were grown on either 10 μg/mL chicken TN-C (n = 4; A,-C,M,Q) or 1 μg/mL (n = 3), 5 μg/mL (n = 3) or 10 μg/mL (n = 3) of human TN-C (D–L,N–Q). Cells were fixed after 72 h in culture and analyzed with ICC using anti-βIII-tubulin to label neurites (white arrows in A–L) which were measured using NeuronJ. Results were analyzed using one-way analysis of variance (ANOVA) and expressed as mean ± SEM. *P < 0.05, ***P < 0.001. Scale bar in (A–L) = 200 μm.