Figure 7.

iPSC-derived hNPCs express deep-layer cortical neuron markers and progenitor cell markers in vivo. The expression of the cortical neuron marker Tbr1 was analyzed using an antibody against Tbr1 demonstrating that a large proportion of the transplanted hNPCs expressed Tbr1 that co-localized within HuNu-positive cells at 4 weeks (A,B, white arrows). This expression was observed in both WT and α9-eYFP hNPCs across all time points (data not shown). Similarly, expression of the progenitor cell marker DCX was retained following transplant shown at 4 weeks (C–E) and at 8 weeks (F–H) post-transplantation within hNCAM-positive projections (white arrows C–H). Scale bar in (A–H) = 150 μm.