Table 1.
Primary antibodies used for Immunocytochemistry (ICC), Immunohistochemistry (IHC) and Western blotting (WB).
| Antibody | Species | Dilution | Supplier | Cat No. | ||
|---|---|---|---|---|---|---|
| ICC | IHC | WB | ||||
| BDNF | Polyclonal, rabbit | 1:200 | - | - | Abcam | AB72439 |
| Ctip2 | Polyclonal, rabbit | 1:500 | - | - | Abcam | Ab70453 |
| DAPI | - | 1:10,000 | 1:10,000 | - | Thermo Scientific™ | 62248 |
| DCX | Polyclonal, rabbit | 1:500 | 1:500 | - | Abcam | AB18723 |
| GFAP | Polyclonal, rabbit | 1:750 | 1:500 | - | DAKO | ZO33429-2 |
| GFP | Polyclonal, rabbit | 1:1,000 | 1:1,000 | 1:1,000 | Invitrogen | A11122 |
| HuNu | Monoclonal, mouse | - | 1:500 | - | Millipore | MAB1281 |
| Iba-1 | Polyclonal, rabbit | - | 1:500 | - | Wako | 019–19741 |
| MBP | Monoclonal, rabbit | 1:50 | - | - | Cell signaling | D8 × 4Q |
| hNCAM | Monoclonal, mouse | - | 1:200 | - | Santa Cruz, Insight | SC-106 |
| PKCγ | Polyclonal, rabbit | - | 1:200 | - | Santa Cruz, Insight | SC-211 |
| Tbr1 | Polyclonal, rabbit | 1:200 | 1:400 | - | Abcam | AB31940 |
| TN-C | Monoclonal, rabbit | 1:250 | 1:250 | - | Abcam | AB108930 |
| TrkB | Polyclonal, rabbit | 1:1,000 | - | - | Santa Cruz Biotechnology | SC-12 |
| α9-integrin | Polyclonal, rabbit | 1:1,000 | - | 1:1,000 | Thermo Scientific™ | PA5–27771 |
| β-actin | Monoclonal, mouse | - | - | 1:10,000 | Sigma | A5441 |
| βIII-tubulin | Monoclonal, mouse | 1:1,000 | - | - | Sigma | T8660 |
| βIII-tubulin | Polyclonal, rabbit | 1:1,000 | - | - | Covance | MRB-435P |
| β1-integrin | Monoclonal, mouse | 1:500 | - | 1:1,000 | BD Transductions | 610467 |
| Vinculin | Monoclonal, mouse | 1:200 | - | - | Millipore | MAB3574 |