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. 2019 Mar;25(3):292–304. doi: 10.1261/rna.067983.118

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© 2019 van der Horst et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article, published in RNA, is available undera Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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FIGURE 3. — Ribosome footprint data confirms translation of uCCRs. (A,C,E) RNA-sequencing results from Merchante et al. (2015), from total RNA (top) or ribosome footprints (middle), and the mRNA architecture (bottom), where the thicker bars represent the ORFs with the discovered uCCR in red, the thinner bars indicate other regions on the transcript, and the lines represent introns from three different genes: AT5G36250 (A,B), AT4G03260 (C,D), and AT1G01060 (LHY) (E,F). The red bar in panel E indicates a uCCR that is out of frame with the mORF. (B,D,F) Sequence alignments of the discovered uCCRs, aligned using MAFFT v. 7.307 (FFT-NS-2) and displayed using Jalview v. 2.10. See Supplemental Table S2 for species abbreviations.