FIGURE 5.

NRDE2 is required for centrosome maturation, integrity, and function. (A–D) MDA-MB-231 cells were fixed and stained with the indicated antibodies 4 d post-transfection. Maximum intensity z-projections of representative image stacks are shown. Mean foci intensities were measured in a 1–2 µm² circle (depending on the marker) drawn around the brightest point of each marker. More than 20 foci were quantified per condition per replicate (n ≥ 3). (E, left) Maximum intensity z-projections of confocal image stacks of mitotic MDA-MB-231 cells fixed and stained with the centriole marker centrin-2 4 d post-transfection. (Right) Quantification of centriole number and the distance between centriole pairs; at least 70 centriole pairs were analyzed per condition. All intensity and distance measurements were performed using the Zen Blue software (Zeiss). Bar = 5 µm. n.s, not significant. (*) P < 0.05, two-tailed t-test.