Figure 8.

(A) Predicted secondary structure of ELVd-ml(+). In this ribozyme-containing member (335 nt) of the family Avsunviroidae the sequences involved in the hammerhead structures are delimited by flags, motifs conserved in natural hammerhead structures are denoted by bars, and self-cleavage sites are marked by arrows. Adapted from [32]. (B) AFM images of ELVd-ml(+) renatured in the absence of Mg²⁺. A field of 275 × 275 nm is shown on the left panel and two characteristic molecules are zoomed on the right. 3D views of the imaged viroid RNAs, as well as profiles with their measured lengths are also displayed.